Words similar to ority l
Example sentences for: ority l
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These were cultured for 2 wk (stage 3) in a 1:1 mixture of low-glucose DMEM and F-12 media containing 100 mg/l apo-transferrin (Sigma), 288 mg/l glucose, 73 mg/l L -glutamine, 1.69 g/l sodium bicarbonate, 25 μg/ml bovine insulin (Sigma), 20 nM progesterone, 100 μM putrescine, 30 nM sodium selenite, and penicillin/streptomycin.
Similarly, comparison of the alginate producer FRD-1 with FRD-2, its spontaneous nonmucoid derivative, revealed that both strains had very similar C L and MAC values.
Frozen lung tissue was homogenized in ice cold 750 μl radioimmunoprecipitation assay (RIPA) buffer (1×PBS, 1% Igepal CA-630, 0.5% sodium deoxycholate, 0.1% SDS) plus protease inhibitors (30 μl/ml RIPA aprotinin, 10 μl/ml RIPA 100 mmol/l sodium orthovanadate).
The corresponding numbers in L6-S1 were 17.
All crystallographically characterized metallo-β-lactamases have a flexible amino acid chain that extends over the active site [ 37 42 44 45 46 47 48 49 ] . Previous NMR studies on CcrA have shown that this loop "clamps down" on substrate or inhibitor upon binding, and there is speculation that the distortion of substrate upon clamping down of the loop may drive catalysis [ 50 ] . The crystal structure of L1 showed that there is a large loop that extends over the active site, and modeling studies have predicted that two residues, Ile164 and Phe158, make significant contacts with large, hydrophobic substituents at the 2' or 6' positions on penicillins, cephalosporins, or carbapenems [ 37 ] . To test this prediction, Ile 164 and Phe158 were changed from large, hydrophobic residues to alanines to afford the I164A and F158A mutants.
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